Product

Frequently Asked Questions (FAQs)

Product

The Bode Buccal DNA Collector is supported by all major manufacturers’ (Thermo Fisher Scientific, Promega, and QIAGEN) direct amplification kits. Bode Cellmark’s technical services team can provide recommended protocols and cycle numbers to achieve a high first-pass success rate.

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Yes. The Buccal DNA Collector has been treated with an electron beam, making it safe for use in your mouth.

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The Bode Buccal DNA Collector is a direct collection system. The sample processed in the laboratory is the same sample that was collected from the individual’s mouth. As a direct collection system, it does not require the transfer step that is necessary when using treated cards.

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Buccal collection is a non-invasive DNA collection method that eliminates risks associated with handling and transferring blood samples. Additionally, the process of collecting, processing and storing is significantly less expensive when using buccal samples.

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The Bode Buccal DNA Collector should be stored in a cool, dry environment.  A desiccated storage system is recommended for storage in order to control humidity.

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The Bode Buccal DNA Collector is composed of 100% cotton filter paper. Due to this property, it is compatible with most extraction and quantification kits. Internal testing has been based on QIAGEN extraction methods. Our technical services team can assist clients in optimizing extraction and quantification procedures for Buccal DNA Collector samples.

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A forensic paternity case is one that is required as part of a criminal investigation such as incest or an underage mother or an abandoned infant. Court-ready reports will be issued for all such cases (where a proper chain of custody is provided) and expert testimony will be available to support our written results as well.

System / Protocol

Protocol(s) 

Identifiler® Direct

25 μl

GlobalFiler® Express

15 μl

PowerPlex® 16 HS System

25 μl, 12.5 μl

PowerPlex® 18D System

25 μl, 12.5 μl

PowerPlex® 21 System

25 μl, 12.5 μl

PowerPlex® Fusion System

25 μl, 12.5 μl, 6 μl

Investigator® 24plex GO! Kit

20 μl, 10 μl, 5 μl

 

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The Bode Buccal DNA Collector is compatible with both semi-automated and fully-automated punchers. The Bode Buccal 2 has a collector and card component with a conversation that makes the collector/cassette compatible with automated punchers and liquid handling robots.

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Yes. Collection utilizing the Bode Buccal DNA Collector is a simple process that requires minimal training of collection personnel.

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It is possible to obtain DNA from anywhere on the collection paper, as the entire surface is placed inside an individual’s mouth. The highest DNA yields can be obtained from the top (rounded end) of the collector. The lowest amount of DNA is found at the base.  It is recommended to begin sampling from the upper tip of the collector.

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The preferred sample size depends on the processing method. If performing a standard extraction, a 3.2mm or 4.7mm punch is recommended.  If performing a direct amplification procedure, a 1.2 mm punch is recommended.

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After a sample is collected on the Buccal DNA Collector, a small sample can be punched or cut out of the filter paper. The portion of the collector sampled can then be placed in a 96-well plate or microcentrifuge tube for downstream analysis.

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Yes, Bode Cellmark offers several storage solutions that help to protect the integrity of the sample(s). A customized solution can be created for a client’s specific needs.

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Yes. Bode SecurSwab products can be utilized to collect any type of DNA sample.

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The Bode SecurSwab devices are designed so that the protective tube can serve as a handle.  This allows for a more rigorous collection of stains and better sample collection. 

Since it does not have to be air dried before packaging, it reduces the potential to introduce contamination. Packaging of wet swabs can also lead to transfer of sample to the container, thus reducing the amount of DNA left on the swab for testing and possible degradation of the DNA. The Bode SecurSwab DUO-V swab system ranked number one for both small- and large-volume DNA recovery in the Swab Collection Study conducted by the National Forensic Science Technology Center and funded through the National Institute of Justice.1

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Bode SecurSwab devices include a 100% cotton swab. Due to this property, it is compatible with most extraction and quantification kits. Internal testing has been based on Qiagen extraction methods. Our technical services team can assist clients in optimizing extraction and quantification procedures for SecurSwab Collector samples. 

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No.  The integrated desiccants dry a fully-saturated swab in less than five hours.  By eliminating air drying at the crime scene, the Bode SecurSwab enables more efficient handling of evidence and reduces the risk of contamination caused by exposure to the crime scene and personnel.

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Bode SecurSwab devices are designed with a “break point” that enables the entire swab head to be snapped directly into a microcentrifuge tube. Alternatively, a portion of the swab can be cut using clean scissors and placed into a tube for extraction.

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The Bode SecurSwab product line is a collection system that provides sample collection, drying, and protection during transport--all in one device. These unique properties aid in recovering more DNA, as compared to generic swabs, and protecting the sample from degradation and contamination.

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The Bode SecurSwab products can be used to collect forensic evidence at a crime scene or reference samples from individuals for DNA identification purposes.

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It is recommended that the sample be stored in a cool, dry environment.

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