Private laboratories like Bode Cellmark do not have the ability to upload profiles to CODIS. However, we routinely provide state labs with the relevant data that enables profiles generated at Bode Cellmark to be uploaded to CODIS by the relevant agency.
We would recommend sending the femur if available. If the femur is not an option, then a molar or the next largest load-bearing bone available would be the best option. Bode Cellmark has been successful with obtaining full DNA profiles from highly-compromised bone fragments, including victims from Hurricane Katrina and the attack on the World Trade Center.
Mitochondrial DNA Sequencing
Nuclear DNA Analysis
Y-STR testing is beneficial in the case of a mixture with a major component female STR profile and a few alleles from a male contributor. A partial to full Y-STR profile would most likely be obtained because the Y-STR primers can focus on the male DNA without the female DNA interfering with the interpretation. Y-STR testing can be useful for samples believed to have originated from azospermic or vasectomized males, as well as non-sexual samples such as mixed DNA profiles from touch evidence tested from fingernails, clothing, and guns.
Bode Cellmark offers STR amplification using commercially available kits for the generation of the core CODIS 13 loci, including both the Hitachi and Applied Biosystems platforms using Promega’s PowerPlex® 16 System and PowerPlex® 16 BIO System and the Applied Biosystems AmpFLSTR® Profiler Plus®, COfiler®, Identifiler® and MiniFilerTM PCR amplification kits.
LCN is used on items in cases where only minimal contact has occurred or there are only small quantities of cells/DNA present. Unlike touch DNA, these samples are processed through a higher number of polymerase chain reaction (PCR) cycles which may be able to obtain a DNA profile when conventional PCR cannot. LCN is usually only used on a sample that has already been processed and has an indication of the presence of DNA, but cannot be re-worked any further using standard DNA methods.
Bode Cellmark uses a touch evidence procedure for processing samples from areas where you would expect to find DNA (in the form of skin cells) from a person who has come in contact with the item. An example is the handle of a murder weapon or a pair of pants that have been pulled down. The success of touch DNA depends on the sampling method, the type of material, and the shedding capabilities of the suspect, as some people naturally leave behind more skin cells.